Researcher / analyst
The UCSD Department of Cellular and Molecular Medicine (CMM) is one of two basic science units in the School of Medicine where world-class researchers study fundamental cellular processes and pathways using a variety of techniques from classical genetics and fluorescence microscopy to high throughput genomics, systems biology, and crystallography. Our 37 faculty and over 200 postdoctoral scholars, graduate students, and research staff work on the cutting edge of biology, cutting across fields from basic biochemistry and genetics to genomics, systems biology and stem cell biology. The faculty also play a major role in medical and graduate student training, providing educational and programmatic offerings that span several disciplines and provide diversity to meet the interests of a broad spectrum of students and scholars.
The Center for Network Medicine [www.cnetmed.ucsd.edu] is striving towards a goal of understanding the fundamental principles that allow eukaryotic cells to do their business, i.e., iteratively sense, decide, act and learn/adapt. We hypothesize that they do so through an ad hoc molecular interaction network whose architectural principles and computational rules are similar to those used in the layered communication systems in the engineering world. These rules enable noise reduction, error minimization, shielding and encapsulation of breakdowns, control of delays and state explosion, etc. Success in decoding these fundamental rules should not only enable us to complete and 'clean up' the existing incomplete and messy biological networks, but also use these network for simulating and predicting cell behavior. This will usher a new era in network-based diagnostic tools and network-resetting therapeutics for major diseases. On the flip side, knowing how a cell autonomously carries out its business will provide insights into designing new kinds of self-organizing networks (SON) and provide a novel paradigm (beyond artificial neural networks) for developing Artificial Intelligence (AI).
The Stem Cell Research Associate will mostly work within HUMANOID, an organoid-based research core that strives to build disease models for testing therapeutics. Under close supervision, perform standard laboratory procedures with adult human stem cells and primary cells, human pluripotent stem cells (hPSCs), including embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs), as well as other experimental procedures. Perform a number of standard repetitive laboratory procedures with stem cells, primary cell lines and immortalized cell lines, as needed, with the intent to build disease models that will aid in drug discovery for grand challenges in human diseases that are still without cure.
Use basic molecular biology and microscopy techniques to assist in conducting a number of experimental procedures. The research associate will use microscopes, high content imaging, plate-readers, flow cytometers, cell fractionarors, isolators, microfluidic chambers, and available robotics to assay effects of small molecules on stem cells and organoids grown from stem cells will be necessary. The incumbent will assist in performing standard experimental protocols in tissue culture to meet the needs of the research projects, providing ideas and rationale to help improve tests that are unsatisfactory.
Perform general lab duties including ordering supplies and equipment repair.
Under appropriate guidance, the incumbent will edit and maintain database of human tissue and organoids in the HUMANOID biorepository, maintain up-to-date clinic IRBs (Consent forms and IRB protocols), and serve as runner if needed to secure patient-derived tissues from clinics and bring them to the HUMANOID for organoid isolation. For these tasks, the incumbent will need to use word, excel and powerpoint softwares and computers.
Theoretical knowledge of molecular and cellular biology or closely related field as typically acquired at a collegiate level or equivalent combination of education and experience.
Working knowledge of DNA techniques.
Lab experience, knowledge and understanding of sterile techniques and mammalian cell culture practices. Knowledge and experience with numerous types of laboratory equipment related to uses, maintenance, and repair.
Knowledge of small molecule library screening and robotics.
Knowledge of various computer software, hardware, standard office equipment and lab equipment.
Working knowledge and experience in bacteriology and bacteria cell competency.
Ability to use mathematical skills to analyze and develop statistical data.
Knowledge and experience in sterile cell culture techniques for culture of Human Embryonic Stem Cells and other mammalian cells.
Experience performing repetitive and specialized laboratory experimental procedures. Experience following experimental protocols.
Experience using microscopy to perform experiments.
Employment is subject to a criminal background check.
Occasional evenings and weekends may be required.
Work in environment where biological (including infectious agents and blood bourne pathogens) and chemical hazards are present. Research on the human adult and embryonic stem cell. Lentiviral cultures are used.
Must be willing to drive/access public transportation as needed.